将无冰冻保护剂的含夏氏疟原虫（ＰｌａｓｍｏｄｉｕｍＣｈａｂａｕｄｉ）的小鼠肝、脾组织置－４℃、－２６℃和－７６℃冰箱作疟侯虫保种试验。保存于一４℃２ｄ的疟原虫尚能复苏使小鼠感染，保存于一２６℃，１６２ｄ和－７６℃２００ｄ的肝脾组织疟原虫，复苏接种健康小鼠其感染的阳性率分别为６０％和１００％，受染小鼠的血内原虫多在６ｄ－８ｄ出现。实验结果表明，本法保存红内期疟原虫方法简便，效果稳定可靠，保种期长，为一般实验室提供了一种疟原虫保种新方法。 Freezing protective agent containing PlasmodiumChabaudi mice liver and spleen tissue at -4 ℃, -26 ℃ and -76 ℃ freezer for malariae pollinated species test. Plasmodium survived at 4 ℃ for 2 days and then resuscitated the mice to be infected. The plasmodium survived inoculated at 26 ℃, 162d and -76 ℃ for 200 days. The positive rate of infection in healthy mice was 60% And 100% respectively. Most protozoa of infected mice appeared on day 6d-8d. The experimental results show that this method is simple and convenient, the effect is stable and reliable, long-term seed storage, providing a new method for the preservation of malaria parasite in general laboratory.