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目的探讨高脂饮食联合低剂量常用抗生素类药物长期暴露对小鼠葡萄糖和脂质代谢的影响。方法将4周龄的C57BL/6J雄性小鼠随机分成5组,每组8只,分别为高脂对照组(Con)、青霉素低浓度组(5 mg/L)(PL)、青霉素高浓度组(50 mg/L)(PH)、红霉素低浓度组(5 mg/L)(EL)、红霉素高浓度组(50 mg/L)(EH)。用高脂饲料联合药物处理90 d,并在75 d进行糖耐受试验。用HE染色法和油红O染色法观察肝脏组织的病理变化。检测血清游离脂肪酸(FFA)、葡萄糖(Glucose)、三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDLC),以及肝脏中TG的含量,并采用实时荧光定量PCR技术分析肝脏组织中PPARa和PPARg的基因表达水平的变化。结果抗生素药物处理组的肝脏组织切片显示明显的病理变化,肝脏细胞间脂质堆积明显增加,尤其是EH处理组。与高脂对照组相比,抗生素药物处理组的糖耐量异常,血清FFA、Glucose、TG上升(P<0.05),HDL-C呈下降趋势,无显著性。高浓度抗生素药物处理组肝脏中的TG含量和Pparg的表达量都显著高于对照组(P<0.05)。结论青霉素和红霉素低剂量长期暴露导致小鼠肝脏中脂质和糖代谢紊乱。
Objective To investigate the effects of long-term exposure of high-fat diet combined with low-dose antibiotics on glucose and lipid metabolism in mice. Methods Four-week old C57BL / 6J male mice were randomly divided into 5 groups (8 rats in each group): high-fat control group, low-concentration penicillin group (PL), high concentration penicillin group (50 mg / L), low concentration of erythromycin (EL) and high concentration of erythromycin (50 mg / L). The rats were treated with high-fat diet for 90 days, and the sugar tolerance test was conducted on the 75th day. The pathological changes of liver tissues were observed by HE staining and oil red O staining. Serum free fatty acids (FFA), glucose, triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDLC) The changes of gene expression of PPARa and PPARg in liver tissues were analyzed by real-time fluorescence quantitative PCR. Results The liver tissue sections of the antibiotic-treated group showed obvious pathological changes, and the lipid accumulation in the liver cells was significantly increased, especially in the EH-treated group. Compared with the high-fat control group, the antibiotic-treated group had impaired glucose tolerance, increased serum FFA, Glucose and TG (P <0.05), and showed no significant difference in HDL-C. The contents of TG and Pparg in the liver of the high concentration antibiotic treatment group were significantly higher than those in the control group (P <0.05). Conclusion Long-term exposure to low doses of penicillin and erythromycin leads to disorders of lipid and glucose metabolism in the liver of mice.