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目的以铜蓝蛋白(CP)作为大鼠铜摄入量的评价指标,针对不同铜摄入量大鼠血中脂蛋白脂酶(LPL)、肝脂酶(HL)及其与甘油三脂(TG)水平的关系,研究大鼠血中CP活力水平与脂蛋白代谢的关系。方法将56只Wistar大鼠以平均日摄入量(ADI)为基础随机分为7组(对照组,0ADI组,1/25ADI组,1/5ADI组,ADI组,5ADI组,25ADI组),0ADI～25ADI组饲以铜缺乏饲料,铜摄入量分别为0、0.032、0.16、0.8、4、20mg/kg,染毒30d。测定不同铜摄入量大鼠血清中CP、HL、LPL及TG水平,分析不同CP水平大鼠血清中LPL、HL与TG的相关性。结果铜摄入量低于日常摄入量时,大鼠血中CP水平≤20.442U/L,此时大鼠血清中HL活力低于正常水平,差异有统计学意义(P<0.05);当CP水平达到30.279U/L时,各组大鼠血中HL活力与正常对照组差异均无统计学意义(P>0.05)。血中TG水平与HL活力呈线性负相关(R2=0.941,P=0.001)。0ADI～1/5ADI组,LPL活力低于正常对照组,差异有统计学意义(P<0.05);大鼠血中CP活力为37.569U/L时,其LPL活力与正常对照组相比,差异无统计学意义(P>0.05);而ADI组、5ADI组及25ADI组大鼠血中LPL活力均高于正常对照组,差异也有统计学意义(P<0.05)。大鼠血中CP水平在15.912～37.569U/L范围内时,大鼠血中LPL活力随着CP水平的增加而下降。结论随着大鼠血中CP水平的升高,其HL活力与TG水平呈现线性负相关;而当CP水平为20.442～30.279U/L时,LPL活力与血中TG含量保持一致性下降趋势。
Objective To evaluate the effects of ceruloplasmin (CP) on the copper intake in rats and the levels of lipoprotein lipase (LPL), hepatic lipase (HL) and triglyceride TG) levels in rats to study the level of CP in blood and the relationship between lipoprotein metabolism. Methods 56 Wistar rats were randomly divided into 7 groups (control group, 0ADI group, 1 / 25ADI group, 1 / 5ADI group, ADI group, 5ADI group, 25ADI group) on the basis of average daily intake (ADI) 0ADI ~ 25ADI group fed a copper-deficient diet, copper intake were 0,0.032,0.16,0.8,4,20 mg / kg, exposure to 30d. The levels of CP, HL, LPL and TG in serum of rats with different copper intake were determined. The correlations of LPL, HL and TG in sera of rats with different CP levels were analyzed. Results When the copper intake was lower than the daily intake, the level of CP in the blood of the rats was less than 20.442U / L, and the HL activity in the serum of the rats was lower than the normal level at this time (P <0.05). When When the level of CP reached 30.279U / L, there was no significant difference between the HL activity of blood and the normal control group (P> 0.05). There was a linear negative correlation between serum TG level and HL activity (R2 = 0.941, P = 0.001). Compared with the normal control group, the LPL activity in the 0ADI ~ 1 / 5ADI group was lower than that in the normal control group (P <0.05); when the CP activity in the blood of the rats was 37.569U / L, (P> 0.05). However, the levels of LPL in blood of ADI group, 5ADI group and 25ADI group were significantly higher than those of normal control group (P <0.05). When the level of CP in rat blood was between 15.912 and 37.569U / L, LPL activity in rat blood decreased with the increase of CP level. Conclusions With the increase of CP in blood, there is a linear negative correlation between HL activity and TG level. When the CP level is between 20.442 and 30.279 U / L, the LPL activity and blood TG content keep decreasing.