通过肝脏酶解灌流和密度梯度离心法获得的肝窦内皮细胞作为免疫原，建立一株单克隆抗体ＳＥ－Ｓ，它特异性识别大鼠肝窦内皮细胞，而与其它类型的内皮细胞或非内皮细胞无交叉反应。免疫电镜及免疫印迹分析显示，此抗体所识别的抗原（ＳＥ－Ｓ抗原）位于肝窦内皮细胞的膜表面和胞饮小泡的内表面，分子量为４４～４８ｋＤ。以上结果说明ＳＥ－Ｓ抗原与肝窦内皮细胞的特殊功能密切相关。因此，对于研究不同病理、生理状态下的肝脏，ＳＥ－Ｓ可能是一个有用的标志物 The liver sinusoidal endothelial cells obtained by enzymatic digestion of liver and density gradient centrifugation were used as immunogens to establish a monoclonal antibody SE-S, which specifically recognizes rat hepatic sinusoidal endothelial cells, but not with other types of endothelial cells No cross-reaction of endothelial cells. Immunoelectron microscopy and immunoblot analysis showed that the antigen recognized by this antibody (SE-S antigen) was localized on the membrane surface of sinusoidal endothelial cells and the inner surface of vesiculated vesicles with a molecular mass of 44-48 kD. The above results show that SE-S antigen is closely related to the special function of sinusoidal endothelial cells. Therefore, SE-S may be a useful marker for studying the different pathological and physiological states of the liver