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植入前小鼠胚胎（ｐｒｅｉｍｐｌａｎｔａｔｉｏｎ ｅｍｂｒｙｏ）的透明带经软化后，用固定在显微操作臂上的微玻璃针行胚胎分割，２－、４－、８－细胞胚胎及桑椹胚分割成功率分别为９０．６％、８５．０％、７３．６％、７１．４％。所获半胚体外培养后，胚泡发育率分别为６２．１％、８０．１％、８３．０％、８９．２％。随胚胎细胞数目增多，分割成功率降低，而发育率则提高。将８－细胞胚组及桑椹胚组的半胚移植到假孕母鼠后，幼仔出生率分别为１４．６％、１５．１％。同法分割４－细胞人胚胎２个，获半胚４个，分割成功率为１００％。
The zona pellucida of the preimplantation embryo was softened and then divided with embryos of microglass needles fixed on the micromanipulator. The success rates of 2-, 4-, 8-cell embryos and morula were respectively 90.6%, 85.0%, 73.6%, 71.4%. After in vitro culture of the obtained half embryos, the blastocyst development rates were 62.1%, 80.1%, 83.0% and 89.2%, respectively. As the number of embryonic cells increases, the success rate of segmentation decreases, while the rate of development increases. After transplanting the half embryos of 8-cell embryo group and morulae group to pseudopregnant mice, the birth rates of pups were 14.6% and 15.1% respectively. The same method to split 4-cell human embryos 2, obtained by the half embryo 4, segmentation success rate was 100%.