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探讨粉尘螨重组Derf2蛋白对小鼠Treg及特异性标记Foxp3蛋白的干扰作用,从而为粉尘螨抗原治疗弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)提供基础实验支持。通过免疫组化检测Foxp3蛋白在DLBCL患者淋巴结石蜡标本中的表达,利用临床数据对Foxp3蛋白在DLBCL的表达与预后情况进行分析。提取粉尘螨重组Derf2蛋白对A20细胞进行干预,检测A20细胞内Foxp3蛋白表达及对细胞增殖能力的影响。建立小鼠DLBCL成瘤动物模型,对模型鼠注射粉尘螨相关抗原,对比各组动物模型的成瘤时间、成瘤速度及成瘤体积。结果显示,免疫组化检测Foxp3蛋白在DLBCL中的表达,发现瘤组织中Foxp3蛋白表达高于瘤旁组织(P<0.05),通过与DLBCL患者临床病理学特征之间关系的分析,得出瘤组织内Foxp3蛋白的高表达是患者预后不良的因素。注射了粉尘螨抗原的小鼠成瘤速度及体积明显下降(P<0.05),瘤内Foxp3蛋白表达较未注射Derf2减少(P<0.05)。这表明Foxp3蛋白在DLBCL患者体内高表达为预后不良因素之一,粉尘螨Derf2对肿瘤Foxp3蛋白的表达能起干扰作用,并抑制体内肿瘤的生长。
To investigate the interference effect of dermatophagoides pteronyssynctus Derf2 protein on Treg and Foxp3 protein in mice and provide basic experimental support for the treatment of diffuse large B-cell lymphoma (DLBCL) by dust mite antigen. Immunohistochemistry was used to detect the expression of Foxp3 protein in lymph node specimens of DLBCL patients. The clinical data was used to analyze the expression and prognosis of Foxp3 protein in DLBCL. The recombinant Derf2 protein of Dermatophagoides farinae was extracted to interfere with A20 cells, and to detect the expression of Foxp3 protein in A20 cells and its effect on cell proliferation. Mouse DLBCL tumor-bearing animal model was established. Dust mite-related antigens were injected into the model mice. The tumorigenic time, tumor-forming rate and tumor volume of each group were compared. The results showed that the expression of Foxp3 protein in DLBCL was detected by immunohistochemistry. The expression of Foxp3 protein in tumor tissues was higher than that in tumor tissues (P <0.05), and the relationship between Foxp3 protein and clinicopathological features of DLBCL patients showed that tumor The high expression of Foxp3 protein in the tissue is a poor prognostic factor for patients. The mice injected with dust mite antigen had significantly lower rates of tumor formation and volume (P <0.05), and the expression of Foxp3 protein in the tumor was lower than that without Derf2 (P <0.05). This indicates that high expression of Foxp3 protein in DLBCL patients is one of the unfavorable prognostic factors. Dust mite Derf2 can interfere with the expression of tumor Foxp3 protein and inhibit tumor growth in vivo.