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AIM:To study the effects of daidzein on human pancreaticcancer cells in vitro.METHODS:Human estrogen-receptor (ER)-positivepancreatic cancer cells MiaPaCa-2 and ER-negative pancreaticcancer cells PANC-1 were treated by 0.1 μmol/L,1μmol/L,10/μmol/L,25 μmol/L,50 μmol/L,75 μmol/L and 100 μmol/Lof daidzein,respectively.Its antiproliferative effect wasstudied by MTT assay.RESULTS:Daidzein inhibited the growth of MiaPaCa-2and PANC-1 cells at the concentrations from 0.1 μmol/L to100/μmol/L.A dose-and time-dependent manner was found.The IC_(50) of daidzein on MiaPaCa-2 and PANC-1 cells was45 μmol/L and 75 μmol/L,respectively.After MiaPaCa-2 cellswere treated by daidzein for 3 d and at the concentrationsmore than IC_(50),the inhibitory manner was identical and theinhibition appeared a saturation phenomenon,but theinhibitory manner of daidzein on PANC-1 cells was differentfrom that of MiaPaCa-2 cells.CONCLUSION:Daidzein has antiproliferative effects onhuman estrogen-receptor-positive and negative pancreaticcancer cells,but their mechanisms may be different.
AIM: To study the effects of daidzein on human pancreatic cancer cells in vitro. METHODS: Human estrogen-receptor (ER) -positive pancreatic cancer cells MiaPaCa-2 and ER-negative pancreatic cancer cells PANC-1 were treated by 0.1 μmol / L, L, 10 / μmol / L, 25 μmol / L, 50 μmol / L, 75 μmol / L and 100 μmol / L of daidzein, respectively.Its antiproliferative effect wasstudied by MTT assay.RESULTS: Daidzein inhibited the growth of MiaPaCa-2 and PANC -1 cells at the concentrations from 0.1 μmol / L to 100 μmol / L dose-and time-dependent manner was found. IC 50 of daidzein on MiaPaCa-2 and PANC-1 cells was 45 μmol / L and 75 μmol / L, respectively. After MiaPaCa-2 cellswere treated by daidzein for 3 d and at the concentrationsmore than IC 50, the inhibitory manner was identical and the inhibition was a saturation phenomenon, but the inhibition mode of daidzein on PANC-1 cells was differentfrom that of MiaPaCa-2 cells. CONCLUSION: Daidzein has antiproliferativerative effects onhuman estrogen-receptor-positive and negative pancreatic cancer cells, but their mechanisms may be different.