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本试验采用平板溶菌法,测定了新抗生素B—3543对水稻白叶枯病菌的溶菌作用,结果表明其生产菌的分生孢子、菌丝块及抗生素对不同菌系不同菌龄的水稻白叶枯病菌均有很强的溶菌作用,所产生的溶菌圈在一定时间内不断扩大.孢子法所测溶菌圈到14天,直径可达23—35mm;菌块法所测溶菌圈到7天,直径可达30—34.9mm;管碟法所测溶菌圈到4天,直径可达32—34.7mm;纸片法所测溶菌圈到3天,直径可达16.3mm。在此基础上又采用透射电镜对溶菌过程作了显微观察,从细胞形态学角度研究了溶菌作用方式.试验结果表明该素是使细菌细胞壁破裂而导致溶菌,溶菌过程为:①原生质凝结.②细胞壁破裂和原生质外溢。③细胞解体。
In this experiment, the bacteriolytic effect of the new antibiotic B-3543 against Xanthomonas oryzae was determined by the plate-lysis method. The results showed that the conidia, mycelium and antibiotics of the producing bacteria were sensitive to different bacterial strains of rice white leaf Bacteria are bacteria have a strong role in the bacteriolysis, resulting in a certain period of time to expand the bacteriolytic.Lycosis measured by the bacteria circle to 14 days, the diameter of up to 23-35mm; Diameter up to 30-34.9mm; tube plate method measured by the bacterial lysozyme to 4 days, the diameter of up to 32-34.7mm; measured by the bacterial lysozyme disk method to 3 days, the diameter of up to 16.3mm. On this basis, the bacteriolytic process was observed microscopically by TEM, and the bacteriolytic mode was studied from the aspect of cell morphology.The results showed that the bacteriolytic bacterium caused by rupture of bacterial cell wall resulted from the bacteriolytic process as follows: ① coagulation of protoplasm. ② cell wall rupture and spillover. ③ cell disintegration.