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目的:探讨不同年龄儿童外周血白细胞端粒长度及端粒酶活性与儿童先心病发病机制的相关性。方法:采用实时荧光定量PCR检测先天性心脏病2个年龄组及健康受试者外周血白细胞的端粒长度及hTERT mRNA(端粒酶催化亚基)。比较相同年龄的先天性心脏病与健康受试者外周血白细胞的端粒长度及hTERT mRNA差异,并比较不同年龄组间外周血白细胞的端粒长度及hTERT mRNA差异。结果:先天性心脏组3-6岁组的受检者及健康受试者3-6岁组平均外周血白细胞端粒(1.63±0.61)长于先天性心脏组7-10岁组的受检者及健康受试者7-10岁组(1.36±0.46)(t=11.37,P<0.05);各组均无端粒酶表达。结论:外周血白细胞的端粒长度随着年龄的增长而缩短。端粒酶活性与先天性心脏病发病并无直接相关性。
Objective: To investigate the relationship between telomere length and telomerase activity in peripheral blood leukocytes and the pathogenesis of CHD in children of different ages. Methods: Telomere length and telomerase reverse transcriptase (telomerase catalytic subunit) of peripheral blood leukocytes in congenital heart disease patients and healthy subjects were determined by real-time fluorescence quantitative PCR. The telomere length and hTERT mRNA of peripheral blood leucocytes in congenital heart disease and healthy subjects of the same age were compared. The telomere length and hTERT mRNA difference of peripheral blood leucocytes in different age groups were compared. Results: The average number of peripheral white blood cell telomeres (1.63 ± 0.61) in 3-6-year-old congenital heart group patients and 3-6-year-old healthy subjects was longer than that in congenital heart group 7-10-year-old subjects And healthy subjects (1.36 ± 0.46) (t = 11.37, P <0.05). There was no telomerase expression in each group. Conclusion: The telomere length of peripheral white blood cells shortens with age. Telomerase activity and congenital heart disease is not directly related to the incidence.