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1978～81年,作者对成都地区的机关干部和中小学生6479人进行了血红蛋白病的普查,发现了几种异常血红蛋白(Hb)并进行了化学结构分析,现报道如下. 方法先取末梢血,用简快Hb电泳法(1)筛选,有异常Hb区带者进行体检和家族调查,并取静脉血作血红蛋白测定和红细胞形态观察,Hb的醋酸纤维素薄膜和淀粉胶电泳,HbA_2和异常Hb定量,抗硷Hb测定,不稳定性试验(异丙醇试验、热变性试验、变性珠蛋白小体检查),Hb溶解度测定及异常Hb肽链分析(8M尿素解离和醋纤膜肽链电泳法):然后作异常Hb的化学结构分析,即先将Hb溶液制成珠蛋白干粉,经CM—22纤维素柱
From 1978 to 1981, the author conducted a survey of 6,479 institutional cadres and primary and secondary school students in Chengdu for hemoglobinopathies and found several abnormal hemoglobin (Hb) and chemical structure analysis, are reported as follows.Methods Peripheral blood, Simplex Hb electrophoresis (1) screening, abnormal Hb zone were physical examination and family survey, venous blood was taken for hemoglobin determination and erythrocyte morphology, Hb cellulose acetate film and starch gel electrophoresis, HbA 2 and abnormal Hb quantitative , Hb test, instability test (isopropanol test, thermal denaturation test, denatured globin body examination), Hb solubility determination and abnormal Hb peptide chain analysis (8M urea dissociation and vinegar membrane peptide chain electrophoresis ): Then make an analysis of the chemical structure of abnormal Hb, Hb solution first made of haemoglobin powder, the CM-22 cellulose column