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目的 :探讨肺心合剂对野百合碱诱发肺动脉高压大鼠肺血管重建的影响。方法 :利用野百合碱(5 0 mg/ kg)复制大鼠肺心病模型。 6 0只雄性 Wistar大鼠随机分为正常对照组 ,模型组 ,肺心合剂低、中、高剂量组及硝苯地平组 6组 ,每组 10只。分别于制模后 14 d灌胃相应药物 ,连续用药 8d。处死动物后 ,利用特殊染色结合病理图像分析方法测定肺小动脉病理 (光镜下观察和弹力纤维染色切片 )及其形态计量学 (指标为与终末细支气管伴行的肺小动脉管壁中膜厚度、外径、管壁面积、室管腔面积以及与血管外径的比值 )。结果 :肺心合剂及硝苯地平均能明显减轻模型大鼠的肺血管重构 (P均 <0 .0 1) ,以肺心合剂高剂量组效果最好 ;但治疗组各指标仍未完全恢复到正常对照组水平。结论 :肺心合剂能部分逆转肺血管结构重建 ,有效降低肺动脉高压。
Objective : To investigate the effect of Feixin mixture on pulmonary vascular remodeling induced by monocrotaline in rats with pulmonary hypertension. METHODS: Rat pulmonary heart disease model was replicated using monocrotaline (50 mg/kg). Sixty male Wistar rats were randomly divided into normal control group, model group, low-, medium-, and high-dose groups of Feixin mixture and nifedipine group, 6 in each group. The drugs were intragastrically administered on the 14th day after the model was administered and the drug was administered continuously for 8 days. After the animals were sacrificed, the pathology of the pulmonary arterioles (observed by light microscopy and stained with elastic fibers) and their morphometrics were measured by special staining combined with pathological image analysis (index was in the wall of pulmonary arterioles accompanied by terminal bronchioles). Membrane thickness, outer diameter, wall area, lumen area, and ratio to the outer diameter of the vessel). Results: The combination of Feixin Mixture and Nifedipine can significantly reduce the pulmonary vascular remodeling in model rats (P<0.01). The effect of Feixin Mixture in high dose group is best, but the indexes in the treatment group are still not complete. Restore to normal control level. Conclusion: Feixin Mixture can partially reverse pulmonary vascular structural reconstruction and effectively reduce pulmonary hypertension.