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用逆转录－ＤＮＡ聚合酶链式反应（ＲＴ－ＰＣＲ）从１例中国庚型肝炎（ＨＧＶ）患者血清中扩增出含Ｅ１前区部分基因及Ｅ１区５＇端共３０９ｈｐ基因片段。对其中包括Ｅ１区９６ｂｐ在内的１２０ｂｐ核苷酸进行了序列分析，发现此区段基因与录入号为Ｕ４４４０２Ｇｅｎｅｂａｎｋ报道的美国发现的ＨＧＶ相关序列具有９３％的同源性，氨基酸的同源性为９８％。Ｇｏｌｄｋｅｙ蛋白质分析程序显示，此区段的Ｅ１区可能存在抗原位点。化学合成可能为抗原位点长约２９个氨基酸的多肽Ｅ１Ｐ１。利用Ｅ１Ｐ１及ＮＳ３区内短肽ＮＳ３Ｐ１包被的ＥＬＩＳＡ方法检测了１２名非甲－戊肝炎患者血清。Ｅ１Ｐ１检出的２份血清（２／１２）中有抗－Ｅ１Ｐ１ＩｇＧ的存在。ＮＳ３Ｐ１捡出的３份血清（３／１２）中有抗－ＮＳ３Ｐ１ＩｇＧ的存在，其中包括Ｅ１Ｐ１检出的两份阳性血清。本结果说明在ＨＧＶＥ１区内至少有一个抗原位点存在。
A total of 309 bp fragment containing the pre-E1 gene and the 5 ’end of E1 region were amplified by reverse transcription-polymerase chain reaction (RT-PCR) from a Chinese patient with hepatomegaly (HGV). Sequence analysis of the 120bp nucleotide including 96bp in E1 region showed that this region had 93% homology with the HGV related sequence in the United States as reported by U44402 Genebank. The amino acid homology was 98%. The Goldkey protein analysis program shows that there may be antigenic sites in the E1 region of this segment. Chemical synthesis may be the antigenic site of about 29 amino acids polypeptide E1P1. Serum was detected in 12 patients with non-hepatitis E virus using the ELISA method of E1P1 and NS3P1 coated NS3 region. The presence of anti-E1P1 IgG in 2 sera (2/12) detected by E1P1. Anti-NS3P1 IgG was present in 3 sera (3/12) from NS3P1, including two positive sera detected by E1P1. This result shows that there is at least one antigenic site in the HGVE1 region.