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我们采用Singh等介绍的方法,用地衣红/阿尔新蓝法鉴定硫酸粘液取得成功,现介绍如下。一、染液准备地衣红染液配制方法:1g地衣红溶于100 ml70％酒精,然后加lml浓盐酸。二、染色方法①常规脱蜡至水;②0.25％高锰酸钾氧化5min;③0.25％硫酸处理1min;④2％草酸脱色,自来水清洗;⑤地衣红染液浸染4h;⑥在1％盐酸酒精中分化数秒钟,切片再次用水清洗(经分化清洗步骤后要放在显微镜下观察切片背景和着色情况);⑦染1％阿尔新蓝(PH2.5)1min;⑧脱水、透明、封固。
We used the method described by Singh et al. to identify the sulphuric acid mucus with the Lichen Red/Alcian Blue method. The results are as follows. First, the dye preparation Lichen dye preparation method: 1g lichen red dissolved in 100 ml 70% alcohol, then add lml concentrated hydrochloric acid. Second, the dyeing method 1 conventional dewaxing to water; 20.25% potassium permanganate oxidation 5min; 30.25% sulfuric acid treatment 1min; 42% oxalic acid bleaching, tap water cleaning; 5 Lichen red dye liquid dip 4h; 6 in 1% Differentiation of alcohol in hydrochloric acid for a few seconds, the slices were washed with water again (after the differentiation and washing steps, the background and coloring of the slices were observed under a microscope); 7 were dyed with 1% Alcian Blue (PH2.5) for 1 min; 8 dehydrated, transparent, sealed solid.