谷氨酸棒杆菌(Corynebacterium glutamicum)生物合成L-组氨酸主要受三磷酸腺苷(ATP)磷酸核糖基转移酶HisG的L-组氨酸反馈抑制调节,消除这种反馈抑制对于该组氨酸产生菌的生长是首要的一步。为此,本研究组合应用随机突变和酶的理性再设计,得到了在HisG C-末端调控结构域发生突变的突变株,其反馈抵御能力增加,可自发抵抗L-组氨酸类似物β-(2-噻唑)-DL-丙氨酸(2-TA)的毒性。删除整个C-末端的调 The biosynthesis of L-histidine by Corynebacterium glutamicum is mainly regulated by L-histidine feedback inhibition of HisG, an adenosine triphosphate (ATP) phosphoribosyltransferase HisG, eliminating this feedback inhibition for this histidine-producing bacterium The growth is the first step. Therefore, in this study, a combination of random mutagenesis and rational redesign of the enzyme was carried out to obtain a mutant with a mutation in the HisG C-terminal regulatory domain. The mutant has the feedback resistance capability and can spontaneously resist the L- (2-thiazole) -DL-alanine (2-TA). Delete the entire C-terminal tune