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目的探讨磁共振波谱(MRS)揭示放射性脑损伤的细胞代谢改变及病理早期动态演变价值。材料与方法将成年SD大鼠25只随机分为对照组、10Gy、20Gy、30Gy单次照射组和30Gy分次(每周1次,每次10Gy)照射组,用6MeV电子线作垂直全脑照射,各组大鼠于照射后1个月均行MRI及1HMRS检查,分析NAA、Cho和Cr等信号强度改变,以积分面积进行比较,计算NAA/Cr、Cho/Cr和NAA/Cho。各组大鼠在行1HMRS检查后,立即断头处死取大脑后分两侧大脑半球,一侧大脑半球行常规病理HE染色检查,另一侧行电子显微镜检查。结果所有被检查大鼠的大脑MRI均未见异常表现;各照射组大鼠大脑的NAA/Cr、Cho/Cr和NAA/Cr值与对照组相比均有统计学差异。结论用1HMRS来监测大鼠全脑照射后脑组织内的NAA、Cho和Cr的浓度变化,在形态学发生改变之前就可检测出脑组织的代谢异常,并能反映大鼠全脑照射后早期脑组织内超微结构改变,且对放射性脑损伤的病情发展作出准确的估测。
Objective To investigate the effects of magnetic resonance spectroscopy (MRS) on cellular metabolism and early dynamic evolution of radiation brain injury. Materials and Methods Twenty-five adult Sprague-Dawley rats were randomly divided into control group, 10Gy, 20Gy, 30Gy single irradiation group and 30Gy group (once a week, each 10Gy) The changes of signal intensity of NAA, Cho and Cr were analyzed by MRI and 1HMRS at 1 month after irradiation. NAA / Cr, Cho / Cr and NAA / Cho were calculated by integral area. Rats in each group after 1HMRS examination, immediately decapitated after taking the brain on both sides of the cerebral hemisphere, hemisphere side of the routine pathological HE staining, the other side of electron microscopy. Results The brain MRI of all the rats examined showed no abnormality. The NAA / Cr, Cho / Cr and NAA / Cr of the brain in each irradiation group were significantly different from those in the control group. Conclusion 1HMRS can be used to monitor the concentration of NAA, Cho and Cr in brain tissue after whole brain irradiation. The metabolic abnormalities of brain tissue can be detected before morphological changes, Ultrastructural changes within the tissue, and the prognosis of radiation brain injury to make an accurate estimate.