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目的了解脑脊液沉渣瑞氏染色下新型隐球菌的形态学特征,提高对新型隐球菌的检出率。方法采集患者脑脊液标本,用离心沉淀法收集沉渣涂片,瑞氏染色后镜检观察菌体形态特征。结果新型隐球菌在瑞氏染色下有其形态学特征,用此方法诊断2例新型隐球菌脑膜炎。结论脑脊液沉渣瑞氏染色检查对新型隐球菌的检查有一定的诊断价值,可快速诊断新型隐球菌感染。
Objective To understand the morphological features of Cryptococcus neoformans under the Wright’s stain of cerebrospinal fluid and to improve the detection rate of Cryptococcus neoformans. Methods Cerebrospinal fluid (CSF) samples were collected and sediment smears were collected by centrifugation. Morphological characteristics were observed microscopically by Wright’s stain. Results The Cryptococcus neoformans had its morphological characteristics under Wright’s staining. Two cases of Cryptococcus neoformans meningitis were diagnosed by this method. Conclusion Cerebrospinal fluid sediment Wright’s staining test for detection of Cryptococcus neoformans has a certain diagnostic value, rapid diagnosis of Cryptococcus neoformans infection.