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为研究干扰素γ（ｉｎｔｅｒｆｅｒｏｎγ，ＩＦＮ－γ）对人晶体上皮细胞（ＨＬＥＣ）增殖及胶原合成的影响，采用ＭＴＴ比色法和３Ｈ－脯氨酸参入法研究了不同浓度的γ－干扰素对ＨＬＥＣ增殖活性及胶原合成的作用。结果ＩＦＮ－γ（１００—１０００ｕ）能明显抑制ＨＬＥＣ的增殖和胶原合成，１０００—１００００ｕ能显著对抗１０％小牛血清对ＨＬＥＣ的促增殖作用。结论ＩＦＮ－γ或许可以为临床筛选预防后发性白内障的药物提供依据
In order to study the effect of IFN-γ on the proliferation and collagen synthesis of human lens epithelial cells (HLECs), MTT assay and 3H-proline incorporation assay were used to study the effects of different concentrations of interferon-γ Proliferation of HLEC and the role of collagen synthesis. Results IFN-γ (100-1000u) significantly inhibited HLEC proliferation and collagen synthesis, and 1000-10000u significantly inhibited the proliferation of HLEC induced by 10% fetal bovine serum. Conclusion IFN-γ may provide a basis for clinical screening of drugs for preventing post-cataract