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目的:探讨上皮-间质转化(EMT)及其转录调控因子Twist1在人胚胎前列腺发育过程中的作用及意义。方法:应用免疫组化方法研究EMT的标志性蛋白E-Cadherin、N-Cadherin、Vimentin及转录调控因子Twist1在不同胎龄(25例,16～39周)人前列腺组织中的表达变化情况,分析其与胚胎前列腺发育的关系。结果:E-Cadherin仅表达于前列腺腺体,Vimentin、N-Cadherin和Twist1在腺体和腺体周围间质细胞中均有表达。随胎龄增大,腺体E-Cadherin的表达水平逐渐增强,而Vimentin、N-Cadherin和Twist1的表达水平则逐渐减弱。间质表达的Vimentin、N-Cadherin和Twist1在整个过程中无明显变化。结论:人胚胎前列腺发育过程中存在EMT现象,在早期可能有助于前列腺上皮细胞的游走迁移,以形成腺管;后期则可能对上皮细胞的分化起重要调控作用。
Objective: To investigate the role and significance of epithelial-mesenchymal transition (EMT) and its transcriptional regulator Twist1 in the development of human embryonic prostate. Methods: Immunohistochemical method was used to study the expression changes of EMT markers E-Cadherin, N-Cadherin, Vimentin and Twist1 in human prostate tissue with different gestational age (25 cases, 16-39 weeks) Its relationship with embryonic prostate development. RESULTS: E-Cadherin was only expressed in the prostate gland. Vimentin, N-Cadherin and Twist1 were both expressed in the glandular cells and in the glandular cells. With the increase of gestational age, the expression of E-Cadherin in the gland gradually increased, while the expression of Vimentin, N-Cadherin and Twist1 gradually decreased. Interstitial expression of Vimentin, N-Cadherin and Twist1 in the whole process did not change significantly. CONCLUSION: The EMT phenomenon exists in the process of human embryonic prostate development, which may contribute to the migration and migration of prostate epithelial cells in the early stage to form the gland duct. In the late stage, EMT may play an important regulatory role in the differentiation of epithelial cells.