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采用脂质体转染法将人中性粒细胞防御素（ Ｈ Ｎ Ｐ１ ）的 ｃ Ｄ Ｎ Ａ 重组真核表达质粒ｐ Ｂａｂｅ Ｎｅｏ Ｈ Ｎ Ｐ１导入无血清培养的人和兔的气管粘膜上皮细胞，利用逆转录聚合酶链反应（ Ｒ Ｔ Ｐ Ｃ Ｒ）法，在核酸水平检测 Ｈ Ｎ Ｐ１ 在气管上皮细胞的表达。结果：在人和兔的转染上皮细胞中均可检测到 Ｈ Ｎ Ｐ１ｍ Ｒ Ｎ Ａ 的表达，而在未转染的上皮细胞中 Ｒ Ｔ Ｐ Ｃ Ｒ检测结果呈阴性。这一结果与作者先前用免疫组化法在蛋白质水平上检测的结果一致，并证明ｐ Ｂａｂｅ Ｎｅｏ Ｈ Ｎ Ｐ１ 转染至气管粘膜上皮细胞后能得到有效表达。
The cD N A recombinant eukaryotic expression plasmid p BabeNeoH N P1 of human neutrophil defensin (H N P1) was transfected into tracheal epithelium of human and rabbit in serum-free culture by lipofection The expression of HNP1 in tracheal epithelial cells was detected at the level of nucleic acid by RT-PCR. Results: The expression of H N P1m R N A was detected in both human and rabbit transfected epithelial cells, whereas the R TPCR test was negative in untransfected epithelial cells. This result was consistent with the previous results of immunohistochemistry on the protein level by the authors and demonstrated that p Babe-Neo-HN P1 can be efficiently expressed after transfection into the tracheal epithelium.