用晶体上皮细胞和同位素标记的毒毛旋花苷(ouabain)为材料,测定牛眼晶体上皮细胞钠钾ATP泵的量。~3H ouabain与钠钾ATP泵结合包括特异性结合(直接与泵结合)和非特异性结合(滞留在细胞间和培养板上)。前者在~3H ouabain达一定浓度时,即能饱和,后者随~3H ouabain浓度升高而升高。经测定,晶体上皮细胞钠钾ATP泵与~3H ouabain结合的最大值为0.6 Pmoles/100,000个细胞,亦就是说,每个细胞约含有3.6×10~6个钠钾ATP泵。 Crystalline epithelial cells and isotope-labeled ouabain were used as materials to determine the sodium-potassium ATP pump in bovine lens epithelial cells. ~ 3H ouabain binds to the sodium-potassium ATP pump including specific binding (directly to the pump) and non-specific binding (residing between the cells and the culture plate). The former at ~ 3H ouabain up to a certain concentration, that is able to saturate, the latter with ~ 3H ouabain concentration increased. The maximum of 0.6 Pmoles / 100,000 cells bound to the ~ 3H ouabain of the sodium-potassium ATP pump of the crystalline epithelial cells was determined to be about 3.6 × 10 ~ 6 sodium-potassium ATP pumps per cell.