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分离并研究金属碳纳米管(m-SWNTs)的光热效应及其对乳腺癌细胞的抑制作用。采用Pm PV(poly[(m-phenylenevinylene)-alt-(p-phenylenevinylene)])从单壁碳纳米管(SWNTs)中分离得到具有金属特性碳纳米管,测定其光热效应,与MCF-7乳腺癌细胞共同培养,在808 nm波长下,用红外光(NIR)以2 W·cm-2的强度照射细胞3 min,MTT比色法测定m-SWNTs对MCF-7细胞的杀灭作用;流式细胞术测定m-SWNTs对MCF-7细胞凋亡的影响。结果表明,用Pm PV可有效分离m-SWNTs,其光热效应明显增强;可以抑制MCF-7细胞的存活率,促进MCF-7细胞凋亡,其效果强于未经分离的SWNTs。通过Pm PV分离的m-SWNTs具有更高的光热转化效率,并且对MCF-7细胞具有更强的杀灭效果。
The photothermal effects of metal carbon nanotubes (m-SWNTs) and their inhibitory effect on breast cancer cells were isolated and studied. Single-walled carbon nanotubes (SWNTs) were separated from Pm PV (poly [(m-phenylenevinylene) -alt- (p-phenylenevinylene)] to obtain carbon nanotubes with metallic characteristics. The photothermal effects were measured and compared with MCF- Cancer cells were co-cultured and irradiated with 2 W · cm-2 of infrared light (NIR) at a wavelength of 808 nm for 3 min. The killing effect of m-SWNTs on MCF-7 cells was determined by MTT assay. Effect of m-SWNTs on apoptosis of MCF-7 cells by cytometry. The results showed that m-SWNTs could be effectively separated by Pm PV, and its photothermal effect was significantly enhanced. It could inhibit the survival rate of MCF-7 cells and promote the apoptosis of MCF-7 cells, which was more effective than that of non-isolated SWNTs. M-SWNTs isolated by Pm PV had higher photothermal conversion efficiency and had a stronger killing effect on MCF-7 cells.