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目的探讨存活蛋白(Livin)、生存蛋白(Survivin)在人肝癌多药耐药(MDR)细胞株Bel-7402/5-Fu中的表达及其与肝癌MDR的关系。方法通过5-Fu药物浓度梯度递增法诱导建立肝癌耐药细胞亚系Bel-7402/5-Fu,Real-time PCR法检测Bel-7402、Bel-7402/5-Fu中Livin、Survivin mRNA的表达,蛋白印迹法检测Bel-7402、Bel-7402/5-Fu中Livin、Survivin蛋白的表达。结果 Bel-7402/5-Fu细胞中Livin、Survivin mRNA和蛋白的表达量均高于Bel-7402细胞。结论 Livin、Survivin的高表达可能是人肝癌细胞MDR形成的原因之一。
Objective To investigate the expression of Survivin and Survivin in Bel-7402/5-Fu human hepatocellular carcinoma multidrug-resistant (MDR) cell line Bel-7402/5-Fu and its relationship with MDR. Methods The expression of Livin and Survivin mRNA in Bel-7402 and Bel-7402/5-Fu cells was detected by Real-time PCR and Bel-7402/5-Fu cell line induced by 5-Fu concentration gradient. The protein expression of Livin and Survivin in Bel-7402 and Bel-7402/5-Fu was detected by Western blot. Results The expression of Livin, Survivin mRNA and protein in Bel-7402/5-Fu cells were higher than that in Bel-7402 cells. Conclusion The high expression of Livin and Survivin may be one of the reasons for the formation of MDR in human hepatoma cells.