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为获得TMV弱毒,在选择轻症纯毒系的基础上,以亚硝酸诱变处理30、40、50、60分钟,得到了30个无症突变株,经室内交叉保护试验,其中DN60-3对TMV的重花叶株系、条斑株系和山东济南的坏疽型都有显著的干扰作用,而对生产影响不大的黄色花叶株系和黄色环斑株系的干扰作用差。田间试验示范结果,对保护地栽培的番茄花叶病(包括卷叶病)的防病增产效果明显。同时发现,栽培技术差,植株长势弱,则保护放果减低。由于弱毒的保护作用属于持异性免疫,因此,在弱毒应用上,对TMV和CMV混发地区的露地番茄无保护作用。随着抗TMV番茄品种的全面推广,弱毒的利用将会失去重要性,目前一些中小城市番茄品种正处于更新时期,弱毒仍可发挥一定作用。
In order to obtain TMV attenuated virus, 30 mutants were obtained by mutagen treatment of nitric acid for 30, 40, 50, and 60 minutes on the basis of selection of mild pure strains. Cross-protection test was conducted in 30 strains, of which, DN60-3 There were significant interference effects on TMV heavy mosaic, streaked lines and gangrenous type in Shandong Jinan, but the yellow mosaic and yellow circular spotted lines which had little effect on the production had a poor interference effect. Field experiment demonstration results show that the disease prevention and yield increase effect of cultivated tomato mosaic disease (including curly leaf disease) in protected areas is obvious. Also found that poor cultivation techniques, plant growth is weak, the protection of putty reduced. As the protective effect of attenuated virus belongs to heterosexual immunity, it has no protective effect on tomato in TMV and CMV mixed area in the field of attenuated virus application. With the full promotion of anti-TMV tomato varieties, the utilization of attenuated drugs will lose its importance. At present, some tomato varieties in small and medium-sized cities are in a renewal period and the attenuated viruses can still play a role.