论文部分内容阅读
目的研究高糖环境下体外培养大鼠海马神经元氧化损伤时乏氧诱导因子-lα(HIF-lα)表达的变化以及脑神康胶囊对神经元的保护作用。方法原代培养大鼠海马神经元,将细胞随机分成8组:对照组、高糖组、黄嘌呤/黄嘌呤氧化酶组(X/XO组)、川芎嗪组、生理盐水组、脑神康低(5mL/kg)、中(10mL/kg)、高浓度组(20mL/kg),对照组用低糖型DMEM培养基培养,其余7组用葡萄糖浓度为25mmol/L的DMEM培养基培养。除对照组及高糖组正常培养,其余各组均用黄嘌呤/黄嘌呤氧化酶处理建立氧化损伤模型,其中中药各浓度组及川芎嗪组分别于加入黄嘌呤/黄嘌呤氧化酶之前换以不同浓度含药血清和川芎嗪的DMEM培养液,采用Real-Time PCR法检测各组海马神经元HIF-1αmRNA的表达。结果与对照组相比,高糖组及X/XO组HIF-lα mRNA表达均明显上升(P均<0.01);与高糖组相比,X/XO组HIF-1α mRNA的表达量升高(P<0.05);与X/XO组比较,川芎嗪组及中药各浓度组HIF-1αmR-NA的表达量均降低,其中中浓度组最低,具有明显的统计学意义(P均<0.01),川芎嗪组与低浓度组接近,无统计学差异(P>0.05)。结论脑神康胶囊对高糖环境下培养的大鼠海马神经元氧化损伤有明显的保护作用,其作用机制可能与其抑制HIF-1α的表达、改善组织缺氧密切相关。
Objective To investigate the changes of hypoxia-inducible factor-1α (HIF-1α) expression in cultured hippocampal neurons cultured in vitro under high glucose conditions and the neuroprotective effects of Naoshenkang capsule on neurons. Methods Primary cultured rat hippocampal neurons were randomly divided into 8 groups: control group, high glucose group, xanthine / xanthine oxidase group (X / XO group), ligustrazine group, saline group, (5mL / kg), medium (10mL / kg) and high concentration group (20mL / kg). The control group was cultured in low glucose DMEM medium and the other 7 groups were cultured in DMEM medium with glucose concentration of 25mmol / L. Except control group and high glucose group, the other groups were treated with xanthine / xanthine oxidase to establish the model of oxidative damage. The concentration of each group and the ligustrazine group were changed before adding xanthine / xanthine oxidase Different concentrations of drug-containing serum and ligustrazine in DMEM culture medium, using Real-Time PCR method to detect the expression of HIF-1αmRNA in hippocampal neurons. Results Compared with the control group, the expression of HIF-1α mRNA in high glucose group and X / XO group was significantly increased (all P <0.01). Compared with high glucose group, the expression of HIF-1α mRNA in X / XO group increased (P <0.05). Compared with the X / XO group, the expression of HIF-1αmR-NA in the Ligustrazine group and the traditional Chinese medicine group were all decreased, , Ligustrazine group and low concentration group close, no statistical difference (P> 0.05). CONCLUSION Naoxinkang capsule can protect rat hippocampal neurons from oxidative damage induced by high glucose. The mechanism may be related to the inhibition of HIF-1α expression and the improvement of hypoxia.