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芥蓝的研究多集中在分类、常规种资源遗传多样性等方面。随着芥蓝杂交种的问世,杂交种的优势越来越突出,市场效果明显,因此,对芥蓝自交系的遗传多样性等研究尤为重要。选取分布在甘蓝9条染色体上的55对SSR引物,从中筛选出多态性好、分布均匀的15条SSR引物对98份芥蓝自交系进行分析,共得到65个多态性带型。采用UPGMA方法构建了一张聚类图,在相似系数为0.71时,将98份芥蓝自交系初步分为1,2,3,4,5组。研究结果为芥蓝育种中的亲本选配、杂交种鉴定和资源保护等提供了依据。在试配芥蓝新组合时,应选择遗传距离较远的自交系作为重点材料,后代的杂种优势会更显著。
Most research on kale concentrates on classification and genetic diversity of conventional species. With the advent of kale hybrids, the advantages of hybrids become more and more prominent, and the market effect is obvious. Therefore, it is particularly important to study the genetic diversity of kale inbred lines. Fifty-five pairs of SSR primers distributed on 9 chromosomes of Brassica oleracea were selected, and 15 SSR primers with good polymorphism and uniform distribution were selected to analyze 98 kale inbred lines. A total of 65 polymorphic bands were obtained. A UPGMA method was used to construct a cluster map. When the similarity coefficient was 0.71, 98 kale inbred lines were divided into 1, 2, 3, 4 and 5 groups. The results provided the basis for parental selection, identification of hybrids and resource protection in kale breeding. When trying on the new combination of Chinese cabbage, the inbred lines with longer genetic distance should be selected as the key materials, the heterosis of the offspring will be more significant.