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目的探讨湖北海棠叶总黄酮对日本血吸虫感染所致小鼠肝纤维化的保护作用。方法随机将未感染和感染日本血吸虫尾蚴昆明小鼠分成A～F6组。即未感染空白对照组(A)、模型组(B)、复方鳖甲软肝片阳性组(C)、湖北海棠叶总黄酮治疗高剂量[114mg(/kgd)]组(D)、中剂量[57mg(/kgd)]组(E)、低剂量[28.5mg(/kgd)]组(F),每组10只。感染后第42天,C、D、E、F组均用吡喹酮驱虫治疗,500mg/(kgd)2日疗法,之后各组开始灌胃给药持续治疗60d,A组和B组生理盐水灌胃对照。实验结束处死小鼠,留取肝脏组织和血清,测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、透明质酸(HA)含量,计算肝体指数,测定肝组织中羟脯氨酸(HYP)。苏木素伊红和Masson胶原纤维染色观察小鼠肝组织变性、虫卵肉芽肿与胶原沉积等病理改变。结果与A组相比,B、C、D、E、F组小鼠肝脏有明显虫卵肉芽肿形成、胶原沉积与肝纤维化,伴有不同程度肝细胞炎性损伤坏死。血清中ALT、AST、HA和肝组织中HYP含量均明显升高(P<0.05或P<0.01)。与B组相比,湖北海棠叶总黄酮高、中、低剂量组ALT、AST、HA和HYP含量及肝体指数均明显降低(P<0.05或P<0.01),肝组织坏死与胶原沉积明显减轻,虫卵肉芽肿面积减小。结论湖北海棠叶总黄酮具有抗血吸虫性肝纤维化作用。
Objective To investigate the protective effect of total flavonoids from Begonia Caulis L. on hepatic fibrosis induced by Schistosoma japonicum in mice. Methods Kunming mice were randomly divided into A ~ F6 group and non-infected and infected with Schistosoma japonicum cercariae. That is, the group without infection (A), the model group (B), Fufang Biejia Ruangan Tablet positive group (C), Hubei Begonia total flavonoids treatment of high dose [114mg (/ kgd) [57mg (/ kgd)] group (E), low dose [28.5mg (/ kgd)] group (F), 10 mice in each group. On the 42nd day after infection, all the mice in groups C, D, E and F were treated with praziquantel for detoxification and 500 mg / (kgd) for 2 days. After that, the mice in each group were treated with gavage for 60 days. Groups A and B Saline irrigation control. The mice were sacrificed at the end of the experiment, liver tissue and serum were collected, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and hyaluronic acid (HA) were measured, liver index, Hydroxyproline (HYP) in liver tissue was measured. Hematoxylin eosin and Masson collagen fibers were stained to observe the pathological changes of liver degeneration, granuloma and collagen deposition in mice. Results Compared with group A, the liver of mice in groups B, C, D, E and F had obvious formation of egg granuloma, collagen deposition and liver fibrosis with inflammatory necrosis of hepatocytes to varying degrees. Serum ALT, AST, HA and liver tissue HYP levels were significantly increased (P <0.05 or P <0.01). Compared with group B, the levels of ALT, AST, HA and HYP and the index of hepatic body of high, medium and low dose of Begonia officinalis leaf in Hubei Begonia were significantly decreased (P <0.05 or P <0.01), necrosis of liver tissue and collagen deposition were obvious Reduce the area of the egg granuloma reduced. Conclusion The total flavonoids of Begonia officinalis L. has anti-schistosome effect of hepatic fibrosis.