正常小鼠肝脏能贮溜大量HpD,当用320～350J/cm~2剂量的激光照射时即发生光敏效应。肝细胞最早发生改变的是线粒体和糖原,继而内质网、溶酶体、细胞核及血窦面的细胞膜等也发生明显的变性坏死变化。血窦内的枯否细胞变化也很明显,内皮细胞也相继发生改变。从实验结果表明,光敏效应在细胞的膜结构、核染色质等都起反应,支持单态氧学说,并为临床应用提出了应注意的问题。 Normal mouse liver can store a large number of HpD storage, when the use of 320 ~ 350J / cm ~ 2 dose of laser irradiation occurs when the photosensitive effect. The earliest changes in liver cells are mitochondria and glycogen, followed by endoplasmic reticulum, lysosomes, nuclei and sinusoidal cell membrane also occurred obvious degeneration and necrosis changes. Serum sinusoidal Kupffer cells changes are also obvious, endothelial cells have also changed. The experimental results show that the photosensitive effect reacts in the cell membrane structure and nuclear chromatin, supports the theory of singlet oxygen, and puts forward the problems that should be noticed for the clinical application.