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目的研究前列腺癌中增殖细胞核抗原(PCNA)表达及AgNOR计数的意义。方法应用免疫组织化学方法和银染色技术检测前列腺癌和良性前列腺增生组织中PCNA表达和AgNOR计数。结果PCNA增殖指数与AgNOR计数在癌组织中均明显高于良性前列腺增生组织(P均<0.001),且两者均与肿瘤组织学分级和预后有密切关系(P均<0.01和P均<0.001);前列腺癌PCNA增殖指数与AgNOR计数间存在非常显著的正相关(P<0.01)。结论PCNA增殖指数和AgNOR计数结合分析,在鉴别前列腺良恶性病变和判断前列腺癌的恶性程度及预测患者预后方面具有十分重要的意义。
Objective To study the expression of proliferating cell nuclear antigen (PCNA) and the significance of AgNOR in prostate cancer. Methods Immunohistochemistry and silver staining were used to detect PCNA expression and AgNOR count in prostate cancer and benign prostatic hyperplasia tissues. Results PCNA proliferation index and AgNOR count were significantly higher in benign prostatic hyperplasia tissues than in benign prostatic hyperplasia tissues (all P <0.001), and both were closely related to tumor grade and prognosis (all P <0. 01 and P <0.001). Prostate cancer PCNA proliferation index and AgNOR count had a significant positive correlation (P <0.01). Conclusions The combination of PCNA proliferation index and AgNOR count is of great significance in differentiating benign and malignant prostatic lesions, judging the malignant degree of prostate cancer and predicting the prognosis of patients.