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目的:探讨端粒酶(hTERT)启动子驱动的肿瘤坏死因子相关凋亡诱导配体(TRAIL)对宫颈癌细胞生长抑制的作用。方法:脂质体转染将hTERT-TRAIL转染至宫颈癌细胞HeLa,RT-PCR检测TRAILmRNA的表达;通过MTT、流式细胞术检测稳定转染细胞的生长情况,电镜观察转染细胞的超微结构。结果:转染真核表达载体hTERT-TRAIL组细胞中TRAILmRNA细胞表达量高于对照组(P<0.05);hTERT-TRAIL组细胞对宫颈癌细胞的抑制率高于CMV-TRAIL组和对照组(P<0.05);hTERT-TRAIL组细胞对宫颈癌细胞的凋亡率明显高于转染CMV-TRAIL组和对照组(P<0.01);电镜结果显示,hTERT-TRAIL对细胞作用是以细胞凋亡为主。结论:hTERT启动子驱动的肿瘤坏死因子相关凋亡诱导配体对人宫颈癌细胞的生长有明显的抑制作用和诱导凋亡作用,为宫颈癌的研究奠定基础和临床治疗提供思路。
Objective: To investigate the effect of telomerase (hTERT) promoter-driven tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on the growth of cervical cancer cells. METHODS: The hTERT-TRAIL was transfected into cervical cancer cell HeLa by lipofectamine 2000. The expression of TRAIL mRNA was detected by RT-PCR. The growth of transfected cells was detected by MTT and flow cytometry. microstructure. Results: The expression of TRAIL mRNA in cells transfected with eukaryotic expression vector hTERT-TRAIL was higher than that in control (P <0.05). The inhibitory rate of hTERT-TRAIL in cervical cancer cells was higher than that in CMV-TRAIL and control P <0.05). The apoptosis rate of cervical cancer cells in hTERT-TRAIL group was significantly higher than that in CMV-TRAIL group and control group (P <0.01). The results of electron microscopy showed that the effect of hTERT-TRAIL on cell apoptosis Death-based. Conclusion: hTERT promoter-driven tumor necrosis factor-related apoptosis-inducing ligand can significantly inhibit the growth of human cervical cancer cells and induce apoptosis. It provides a basis for the study of cervical cancer and clinical treatment.