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建立HPCE手性分离琥珀酸美托洛尔和酒石酸美托洛尔对映体的方法。采用HPCE-CZE模式,在220 nm波长下,以β-CD为手性试剂,成功分离了β1受体阻滞剂琥珀酸美托洛尔和酒石酸美托洛尔对映体。考察了影响样品手性分离的主要因素:缓冲液种类、p H、浓度、分离电压、手性试剂的选择等,最终得到可以同时分离两种美托洛尔盐的电泳条件:用125 mmol/L,p H9.3的硼砂-Na OH溶液(含10 mmol/Lβ-CD,体积分数2%甲醇)作为电泳运行液,分离电压20k V。使用上述方法使得琥珀酸美托洛尔和酒石酸美托洛尔的对映体都达到基线分离,分离度分别是2.14和2.23。该方法适用于琥珀酸美托洛尔和酒石酸美托洛尔对映体的分离,亦可作为定量分析的依据。
Establishment of HPCE Chiral Separation of Metoprolol Succinate and Metoprolol Tartrate Enantiomers. Using the HPCE-CZE mode, the enantiomers of metoprolol succinate and metoprolol tartrate were successfully separated using β-CD as a chiral reagent at a wavelength of 220 nm. The main factors influencing the chiral separation of samples were investigated: buffer type, pH, concentration, separation voltage and the selection of chiral reagents. Finally, the electrophoresis conditions for simultaneous separation of two metoprolol salts were obtained: 125 mmol / L, p H9.3 borax-NaOH solution (containing 10 mmol / L β-CD, 2% methanol by volume) was used as the electrophoresis running solution to separate the voltage of 20 kV. The enantiomers of metoprolol succinate and metoprolol tartrate achieved baseline separation using the above method with resolution of 2.14 and 2.23, respectively. The method is suitable for the separation of metoprolol succinate and metoprolol tartrate enantiomers, can also be used as a basis for quantitative analysis.