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目的从胃黏膜微血管形成以及促血管生成因子VEGF、bFGF变化的角度探讨实验性慢性萎缩性胃炎(CAG)伴非典型增生(Dys)发病机理及消痞颗粒对其治疗作用的机制。方法将大鼠随机分为5组,采用综合法复制大鼠CAG伴Dys模型,用免疫组化染色法因子Ⅷ相关抗原抗体显示胃黏膜微血管,免疫组化法显示促血管生成因子VEGF、bFGF在大鼠胃黏膜的表达。结果模型组微血管计数(MVC)、VEGF和bFGF阳性表达较正常组增加,消痞颗粒治疗组三者均明显下降,与模型组比较有显著差异,且疗效优于自然恢复组。消痞颗粒治疗组MVC疗效还优于维酶素治疗组。结论消痞颗粒治疗CAG伴Dys可能通过抑制促血管生成相关因子,减少微血管形成起作用。
Objective To investigate the pathogenesis of experimental chronic atrophic gastritis (CAG) with atypical hyperplasia (Dys) and the therapeutic mechanism of Xiaoying Granules on the changes of gastric mucosal microvascularization and the pro-angiogenic factors VEGF and bFGF. Methods Rats were randomly divided into 5 groups. The rat model of CAG with Dys was synthesized using a comprehensive method. The gastric mucosal microvessels were visualized by immunohistochemical staining with factor VIII-related antigen antibodies. Immunohistochemistry showed that VEGF and bFGF were pro-angiogenic factors. Rat gastric mucosal expression. Results The expression of microvessel count (MVC), VEGF and bFGF in the model group was increased compared with the normal group. The three groups in the Xiaotan granule treatment group were significantly decreased. There was a significant difference compared with the model group, and the curative effect was better than the natural recovery group. The efficacy of MVC in the Xiaotan granule treatment group was also better than that of the riboflavin treatment group. Conclusion Xiaoying Granules may play a role in the treatment of CAG with Dys by inhibiting angiogenesis-related factors and reducing microvascular formation.