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目的 从离子通道水平上探讨晶状体水肿的机制 ,为白内障的病因学研究提供电生理学基础。方法 培养兔晶状体上皮细胞 (LECs) ,采用全细胞膜片钳技术观察LECs静息膜电位和膜上离子通道的活动。结果 培养的LECs静息膜电位为 -17.40mV± 4.67mV(n =15 ) ;LECs膜上存在对低渗敏感的通道 ,在给予 2 5 %低渗液灌流后激活。通道开放具有明显的电压依赖性 ,反转电位为 -5 .5 3mV。此电流可被氯通道特异性阻断剂Tamoxifen( 10 0 μmol/L)明显抑制。结论 LECs膜上存在容量调节性氯通道 ,该通道的启闭异常可能与某些类型晶状体混浊的形成有密切关系
Objective To explore the mechanism of lens edema at ion channel level and provide the basis of electrophysiology for the etiology of cataract. Methods Rabbit lens epithelial cells (LECs) were cultured and whole-cell patch-clamp technique was used to observe the resting membrane potential and ion channel activity in LECs. Results The resting membrane potential of cultured LECs was -17.40mV ± 4.67mV (n = 15). The channels of LECs were sensitive to hypotonicity and activated by 25% hypotonic solution perfusion. Channel opening has obvious voltage dependence, reversal potential -5.33mV. This current was significantly inhibited by the chloride channel-specific blocker Tamoxifen (100 μmol / L). Conclusion There is a capacity-regulated chloride channel in LECs membrane. The abnormality of the channel opening and closing may be closely related to the formation of turbidity of some types of lens