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采用水浴浸取法提取烟草中的糖,以Zorbax糖分析柱(柱温为30℃)、乙腈∶水(80∶20,V/V)为流动相和蒸发光散射检测器分离测定烟草中果糖、葡萄糖、蔗糖和麦芽糖的含量。4种糖在含量80～1000mg/L范围内与其色谱峰面积之间具有良好的线性关系,检出限分别为2.0mg/L,1.6mg/L,1.6mg/L,2.0mg/L。
Tobacco sugar was extracted by water bath leaching method. The fructose content in tobacco was determined by Zorbax sugar column (column temperature 30 ℃), acetonitrile: water (80:20, V / V) as mobile phase and evaporative light scattering detector. Glucose, sucrose and maltose content. There was a good linear relationship between the four kinds of sugars and their chromatographic peak areas in the range of 80-1000 mg / L with the detection limits of 2.0 mg / L, 1.6 mg / L, 1.6 mg / L and 2.0 mg / L, respectively.