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[目的]分析慢性乙型肝炎(CHB)患者血清中抗核抗体(ANA)的分布特征并探讨其与临床相关性。[方法]采用间接免疫荧光法(IIF)检测79例CHB患者和60例健康体检者血清ANA滴度,采用ELISA法和实时荧光定量PCR法分别检测CHB患者HBV血清标志物和HBV-DNA含量。[结果](1)79例CHB患者ANA阳性共30例,总检出率38.0%,60例健康对照ANA阳性共2例,总检出率3.3%,经χ2检验两组间ANA阳性检出率有明显差异(P﹤0.01);(2)CHB患者ANA阳性组与ANA阴性组ALT、AST水平比较,差异无统计学意义;两组患者HBV-DNA定量比较,ANA阳性组高于阴性组(P﹤0.02)。[结论]HBV感染后可诱导机体产生自身免疫反应,患者血清中可检出ANA。HBV复制活跃期伴随有血清ANA滴度增高,提示HBV复制可能促进自身免疫反应。ANA检测在CHB的临床诊断与治疗工作中有一定的指导意义。
[Objective] To analyze the distribution of antinuclear antibody (ANA) in serum of patients with chronic hepatitis B (CHB) and to explore its clinical relevance. [Method] The serum ANA titers of 79 patients with CHB and 60 healthy subjects were detected by indirect immunofluorescence (IIF). The levels of serum HBV DNA and serum HBV DNA were detected by ELISA and real-time fluorescence quantitative PCR respectively. [Results] (1) There were 30 positive cases of ANA in 79 CHB patients, the total positive rate was 38.0%. The positive rate of ANA in 60 healthy controls was 2 and the overall positive rate was 3.3%. ANA was detected byχ2 test (P <0.01). (2) The levels of ALT and AST in ANA-positive group and ANA-negative group were not significantly different between CHB patients. Compared with the ANA-positive group (P <0.02). [Conclusion] HBV infection can induce the body to produce autoimmune reaction, and the serum of patients can detect ANA. HBV replication active phase accompanied by increased serum ANA titer, suggesting that HBV replication may promote autoimmune response. ANA detection in the clinical diagnosis and treatment of CHB has a certain guiding significance.