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多数人线粒体DNA在其序列2650处有一单个PvuⅡ酶切位点[1]。在研究线粒体脑肌病的线粒体DNA基因突变时,常使用PvuⅡ将线粒体DNA线性化,用于Southern杂交检测线粒体DNA基因缺失。我们从一例线粒体脑肌病患者的骨骼肌标本中发现了一个新的人类线粒体DNA的PvuⅡ酶切位点。1材料
The majority of mitochondrial DNA has a single PvuII restriction site at its 2650 sequence [1]. In studying mitochondrial DNA gene mutations in mitochondrial encephalomyopathy, mitochondrial DNA is often linearized with PvuII and used for Southern blotting to detect mitochondrial DNA gene deletions. We found a new PvuII restriction site for human mitochondrial DNA from a skeletal muscle specimen from a patient with mitochondrial encephalomyopathy. 1 material