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目的 采用变性梯度胶电泳方法 (DGGE)研究喂养方式对早产新生儿肠道菌群的影响。方法 收集同期 6对新生儿 1～ 2 1d粪便 ,直接提取细菌总DNA ,扩增 16SrDNAV6～V8区后DGGE分离 ,测序并与EM BL核苷序列数据库进行比较。结果 喂养前肠道菌群类似 ,以梭状芽孢杆菌、链球菌、克雷伯氏菌为主 ,开奶后母乳喂养儿以双歧杆菌为主 ,奶粉喂养儿肠道菌群显示其明显的多态性 ,有双歧杆菌、梭状芽孢杆菌、链球菌、大肠杆菌、克雷伯氏菌、韦荣氏菌、沙雷氏菌以及不经培养细菌。结论 喂养方式对早产新生儿菌群的形成及演替有明显影响 ,PCR -DGGE在多态性 ,动力性 ,菌群的演进变化方面提供了更加准确的数据和补充资料。
Objective To study the effect of feeding mode on the intestinal microflora in neonates with premature labor by denaturing gradient gel electrophoresis (DGGE). Methods Six pairs of feces of newborns were collected for 1 ~ 21 days. Total bacterial DNA was directly extracted and amplified by 16GE rDNAV6 ~ V8. DGGE was isolated, sequenced and compared with that of the EM BL nucleotide sequence database. Results Before feeding, intestinal flora was similar to Clostridium difficile, Streptococcus, Klebsiella dominated, bifidobacterium was the main breastfeeding infants after feeding, and the intestinal microflora in milk powder feeding showed obvious Polymorphisms are Bifidobacterium, Clostridium, Streptococcus, Escherichia coli, Klebsiella, Veillonella, Serratia bacteria and uncultured bacteria. Conclusion Feeding methods have a significant effect on the formation and succession of neonatal neonates. PCR-DGGE provides more accurate data and supplementary data on the polymorphisms, dynamics and evolution of the flora.