论文部分内容阅读
目的:探讨血小板膜糖蛋白Ⅵ(GPVI)和白介素-6(IL-6)在急性缺血性脑卒中早期诊断中的意义。方法:分别采用流式细胞术和ELISA检测缺血性脑卒中患者和健康对照人群GPVI和IL-6水平。通过受试者工作曲线评估GPVI和IL-6的灵敏度、特异度及界值。结果:缺血性脑卒中病人血小板GPVI和血清IL-6水平显著高于对照组,差异有非常显著性(P<0.01)。缺血性脑卒中病人GPVI与IL-6水平呈正相关(P<0.01)。缺血性脑卒中病人GPVI的ROC曲线下面积为0.984,在最适诊断参考值17.76荧光强度(FI)时,诊断的灵敏度为90.00%,特异度为93.33%。IL-6的ROC曲线下面积为0.918,在最适诊断参考值0.55ng/L时,诊断的灵敏度为66.67%,特异度为86.67%。结论:血小板GPVI含量和血清IL-6水平升高与缺血性脑卒中有关。GPVI对缺血性脑卒中具有较高的诊断价值和准确度。
Objective: To investigate the significance of platelet glycoprotein Ⅵ (GPVI) and interleukin-6 (IL-6) in the early diagnosis of acute ischemic stroke. Methods: The levels of GPVI and IL-6 in ischemic stroke patients and healthy controls were detected by flow cytometry and ELISA, respectively. The sensitivity, specificity, and cutoff of GPVI and IL-6 were assessed by subject working curves. Results: The levels of platelet GPVI and serum IL-6 in patients with ischemic stroke were significantly higher than those in the control group (P <0.01). There was a positive correlation between GPVI and IL-6 in ischemic stroke patients (P <0.01). The area under the ROC curve of GPVI in ischemic stroke patients was 0.984. At the optimal diagnostic reference value of 17.76 fluorescence intensity (FI), the diagnostic sensitivity was 90.00% and the specificity was 93.33%. The area under the ROC curve of IL-6 was 0.918. When the optimal diagnostic reference value was 0.55 ng / L, the diagnostic sensitivity was 66.67% and the specificity was 86.67%. Conclusion: Platelet GPVI levels and serum IL-6 levels and ischemic stroke related. GPVI has high diagnostic value and accuracy for ischemic stroke.