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目的:比对UPLC与HPLC法测定金银花药材中木犀草苷的含量,优化测定金银花中木犀草苷含量的方法。方法:建立UPLC和HPLC测定金银花药材中木犀草苷的含量方法。UPLC法为Agilent ZORBAX RH C18(2.1 mm×50 mm,1.8μm),流动相采用乙腈-0.1%磷酸水溶液梯度洗脱,流速0.4 ml·min-1,柱温30℃,检测波长350 nm;HPLC法色谱柱为Agilent ZOTBAX XDB-Phenyl(4.6 mm×250 mm,5μm),流动相乙腈-0.4%冰醋酸溶液梯度洗脱,流速1.0ml·min-1,柱温30℃,检测波长350 nm。结果:2种方法所得含量测定结果一致。HPLC法和UPLC法加样回收率的RSD分别是1.83%和1.24%。结论:UPLC法在缩短分析时间的同时又得到更高的分析灵敏度,大大减少了有机溶剂的消耗,因此UPLC法能够替代HPLC法对金银花中木犀草苷的含量进行测定。
OBJECTIVE: To compare and determine the content of luteolin in honeysuckle by UPLC and HPLC, and optimize the method for determination of luteolin in honeysuckle. Methods: To establish UPLC and HPLC method for the determination of luteolin in honeysuckle. The UPLC method was Agilent ZORBAX RH C18 (2.1 mm × 50 mm, 1.8 μm). The mobile phase was eluted with a gradient of acetonitrile-0.1% phosphoric acid. The flow rate was 0.4 ml · min-1. The column was Agilent ZOTBAX XDB-Phenyl (4.6 mm × 250 mm, 5 μm). The mobile phase was eluted with a gradient of acetonitrile-0.4% glacial acetic acid. The flow rate was 1.0 ml · min -1. The column temperature was 30 ℃ and the detection wavelength was 350 nm. Results: The results obtained by the two methods were consistent. The RSD of HPLC recovery and UPLC sample recovery were 1.83% and 1.24%, respectively. Conclusion: UPLC method can shorten the analysis time and get higher analytical sensitivity, greatly reducing the consumption of organic solvents, so UPLC method can replace the determination of luteolin in honeysuckle content by HPLC.