论文部分内容阅读
目的建立HPLC法同时测定山楂叶提取物中牡荆素-2″-O-鼠李糖苷、金丝桃苷、槲皮素、山奈酚的含量。方法色谱柱为DIAMONSIL C18柱(250 mm×4.6 mm,5μm);流动相为甲醇-体积分数为0.5%乙酸水溶液,梯度洗脱[0～15 min,φ(甲醇)=40%～65%;15～20 min,φ(甲醇)=65%～100%;20～40 min,φ(甲醇)=100%];流速为1.0 mL.min-1;波长为365 nm。结果牡荆素-2″-O-鼠李糖苷、金丝桃苷、槲皮素、山奈酚的线性关系良好,其质量浓度分别在14.0～112 mg.L-1、3.50～28.0 mg.L-1、6.25～50.0 mg.L-1、0.550～4.40 mg.L-1内与峰面积呈良好的线性关系。平均回收率分别为101.3%、98.6%、107.0%、98.1%,RSD分别为1.1%、2.2%、2.6%、2.1%(n=5)。结论该法简便、可行,重现性好,结果准确,可作为山楂叶提取物的含量测定方法,为其质量控制提供参考依据。
OBJECTIVE To establish an HPLC method for simultaneous determination of vitexin-2 “-O-rhamnoside, hyperin, quercetin and kaempferol in hawthorn leaf extract.METHODS The chromatographic column was DIAMONSIL C18 column (250 mm × 4.6 (methanol) = 40% ~ 65%; 15 ~ 20 min, φ (methanol) = 65%, and the mobile phase consisted of methanol with a volume fraction of 0.5% ~ 100%; 20 ~ 40 min, φ (methanol) = 100%] at a flow rate of 1.0 mL.min-1 and a wavelength of 365 nm.Results Vitexin-2 ”-O- rhamnoside, , Quercetin and kaempferol, and their mass concentrations ranged from 14.0 to 112 mg.L-1, 3.50 to 28.0 mg.L-1,6.25 to 50.0 mg.L-1, from 0.550 to 4.40 mg.L -1 within the peak area showed a good linear relationship. The average recoveries were 101.3%, 98.6%, 107.0% and 98.1%, respectively, with RSDs of 1.1%, 2.2%, 2.6% and 2.1%, respectively. Conclusion The method is simple, feasible, reproducible and accurate. It can be used as a method to determine the content of hawthorn leaf extract and provide a reference for its quality control.