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目的研究乙型肝炎病毒(HBV)对外周血单个核淋巴细胞(PBMC)凋亡的作用。方法利用10名健康献血员外周血分离PBMC,分为加HBV阳性血清组和加健康人血清对照组,经培养72h后,采用PI染色法在流式细胞仪上检测细胞的凋亡情况。结果加HBV阳性血清组培养细胞的凋亡率为(39.56±7.03)%,明显高于加健康人血清组培养细胞的凋亡率(27.57±7.78)%,两组间具有非常显著的差异(P∨0.02)。结论HBV可能具有诱导PBMC凋亡的能力,这可能是形成HBV慢性感染免疫耐受的一个重要机制。
Objective To study the effect of hepatitis B virus (HBV) on the apoptosis of peripheral blood mononuclear lymphocytes (PBMC). Methods PBMCs were isolated from peripheral blood of 10 healthy blood donors. The serum was divided into HBV positive serum group and healthy human serum control group. After cultured for 72 hours, the apoptosis of cells was detected by flow cytometry with PI staining. Results The apoptosis rate of cultured cells with HBV positive serum was (39.56 ± 7.03)%, which was significantly higher than that of healthy human serum (27.57 ± 7.78)%. There was a significant difference between the two groups P∨0.02). Conclusion HBV may induce the apoptosis of PBMC, which may be an important mechanism of forming immune tolerance of chronic HBV infection.