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目的探讨活血行气方药物血清治疗人胃癌SGC-7901的作用。方法采用血清药理学方法,制备药物血清,应用MTT法测定细胞增殖抑制率,在光、电镜下观察细胞凋亡的的形态学变化。结果药物血清作用24h,中药小剂量组(5μl/ml)未见明显抑制作用,其余各组(10μl/ml,20μl/ml)均表现出不同程度的抑制作用;作用48h,中药小剂量组出现抑制作用,各药物血清组随着作用时间的延长抑制更明显,抑制效应呈时间依赖性。顺铂的抑制作用强于药物血清。光镜下,HE染色药物血清组细胞,为圆形或卵圆形,核染色质密集。电镜下人胃癌SGC-7901细胞为圆形,微绒毛及突起明显减少,伪足消失,线粒体空泡化,核染色质凝集,可见调亡小体。凋亡细胞百分率与药物浓度呈正相关。结论活血行气方具有明显抑制人胃癌SGC-7901细胞增殖、诱导细胞凋亡的作用,活血行气方与顺铂合用有协同作用。
Objective To investigate the effect of Huoxue Xingqi recipe serum on human gastric cancer cell line SGC-7901. Methods Serum pharmacology method was used to prepare the drug serum. MTT assay was used to determine the cell proliferation inhibition rate. Morphological changes of apoptosis were observed under light and electron microscope. Results The serum of the rats was treated with the drug serum for 24 h and no significant inhibition was observed in the low dose group of Chinese medicine (5 μl / ml). The rest of the groups (10 μl / ml, 20 μl / ml) showed different degrees of inhibition; Inhibition of each drug serum group with the extension of the role of inhibition of more obvious inhibition of the effect of a time-dependent manner. Cisplatin is more potent than drug serum. Light microscope, HE staining drug serum cells, round or oval, dense chromatin. Under the electron microscope, SGC-7901 cells were round, with obvious decrease of microvilli and protuberances, disappearance of pseudopods, vacuolization of mitochondria and agglutination of nuclear chromatin. The percentage of apoptotic cells was positively correlated with drug concentration. Conclusion Huoxue Xingqi recipe can significantly inhibit the proliferation and induce the apoptosis of human gastric cancer SGC-7901 cells. The combination of Huoxue Xingqi Recipe and cisplatin has a synergistic effect.