目的　研究冬凌草中冬凌草甲、乙素的含量测定。方法　采用高效液相色谱法进行测定。结果　冬凌草甲素进样量在0 .196～ 0 .980 μg之间线性关系良好 ,相关系数r =0 .9999,回收率为 99.2 9% ,测定的RSD =2 .1%。冬凌草乙素进样量在0 .1712～ 0 .85 6 0 μg之间线性关系良好 ,相关系数r=0 .9999,回收率为 98.6 3% ,测定的RSD =1.9%。 结论　本法简便、准确、灵敏 ,重现性好 ,可用于冬凌草及其制剂的质量控制。 Objective To determine the content of Rubescens A and B in Rubescens. Methods Determination by high performance liquid chromatography. Results The orographic content of oridonin was good in the range of 0.196～ 0.980 μg. The correlation coefficient was r=0.9999, the recovery was 99.29%, and the RSD determined was 2.1%. There was a good linear relationship between 0.117-12.85 60 μg injection of oridonin, the correlation coefficient r=0.9999, the recovery rate was 98.6 3%, and the measured RSD was 1.9%. Conclusion This method is simple, accurate, sensitive, and reproducible. It can be used for the quality control of Oridonin and its preparations.