我们从1978年起、对培养基中的大量元素、铁盐、微量元素和有机物应用正交设计,对不同因素水平进行试验,建立了一个适合小麦花药培养的 C_17培养基。据不完全统计,国内已有18个省市自治区的30多个科研和教学单位应用了 C_17培养基,占小麦花培育种单位的90%,国外已有美国、德国、西班牙、丹麦、比利时、捷 Since 1978, orthogonal design has been applied to a large number of elements, iron salts, trace elements and organic compounds in culture medium. Different levels of factors have been tested and a C_17 medium suitable for wheat anther culture has been established. According to incomplete statistics, more than 30 research and teaching institutes in 18 provinces, autonomous regions and municipalities have applied C_17 medium, accounting for 90% of the total cultivars of wheat cultivars. Other countries in the world have been such countries as the United States, Germany, Spain, Denmark, Belgium, Czech