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实验发现,重组灵芝免疫调节蛋白(rLz-8)可直接杀伤人急性早幼粒细胞白血病细胞株NB4.利用异硫氰酸荧光素(FITC)标记rLz-8,其相关的活性实验结果和晶体结构分析都表明,FITC没有影响rLz-8已知生物学功能.通过激光共聚焦显微镜观察FITC-rLz-8在NB4细胞内的动态过程发现,FITC-rLz-8可识别细胞膜上的受体,并可进入细胞质,并最终富集在细胞核区域内.Annexin V-FITC双染检测结果显示,rLz-8对NB4细胞杀伤作用的可能机制是对NB4细胞凋亡的诱导作用,在一定浓度范围内,剂量与凋亡诱导率成正相关.因此rLz-8能够诱导肿瘤细胞NB4发生凋亡的亚细胞学机制可定位在细胞核上.
The experiment found that the recombinant ganoderma lucidum immunoregulatory protein (rLz-8) can directly kill the human acute promyelocytic leukemia cell line NB4. The rLz-8 is labeled with fluorescein isothiocyanate (FITC), and its related activity test results and crystals Structural analysis showed that FITC did not affect the known biological functions of rLz-8. The dynamic process of FITC-rLz-8 in NB4 cells was observed by laser confocal microscopy. FITC-rLz-8 recognized the receptors on the cell membrane. And can enter the cytoplasm, and eventually enriched in the nuclear region. Annexin V-FITC double staining results showed that the possible mechanism of rLz-8 killing of NB4 cells is the induction of apoptosis in NB4 cells, within a certain concentration range There is a positive correlation between the dose and apoptosis inducing rate, so the subcellular mechanism that rLz-8 can induce apoptosis of tumor cells NB4 can be located in the nucleus.