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建立了液相色谱-串联质谱法测定大鼠血浆中的利伐沙班(1)。采用C18色谱柱,以艾瑞昔布(2)为内标,乙腈∶10 mmol/L乙酸铵溶液(用甲酸调至p H 3.14)(50∶50)为流动相,ESI源正离子检测方式,多反应监测,监测离子对m/z 436.2→m/z 145.2(1),m/z 370.1→m/z 278.0(2)。SD大鼠经口灌胃给予1,主要药动学参数分别为:c_max(438.19±122.02)ng/ml,t_max(0.69±0.20)h,AUC_0→24 h(1 692.75±654.72)ng·h·ml~-1,AUC0→∞(1 760.25±649.11)ng·h·ml~-1,t_1/2(3.46±1.10)h。
A liquid chromatography-tandem mass spectrometry (LC-MS / MS) was established for the determination of rivaroxaban in rat plasma (1). C18 column was used with elecoxib (2) as an internal standard. Acetonitrile: 10 mmol / L ammonium acetate solution (adjusted to pH 3.14 with formic acid) (50:50) was used as the mobile phase. ESI source positive ion detection , Multiple reaction monitoring, monitoring ion pair m / z 436.2 → m / z 145.2 (1), m / z 370.1 → m / z 278.0 (2). The main pharmacokinetic parameters were as follows: c_max (438.19 ± 122.02) ng / ml, t_max (0.69 ± 0.20) h, AUC_0 → 24 h (192.75 ± 654.72) ng · h · ml ~ -1, AUC0 → ∞ (1 760.25 ± 649.11) ng · h · ml ~ -1, t_1 / 2 (3.46 ± 1.10) h.