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采用含ｎｏｓ基因和人工合成柞蚕抗菌肽Ｄ基因ＡＰ－Ｄ基因（含ＣａＭＶ３５ｓ启动子）的根癌农杆菌对沙田柚外植体进行转基因研究，与根癌农杆菌共培养产生的芽、苗经Ｋｍ敏感性筛选，获得了若干转基因植株，并对这些植株进行了报告基因ｎｏｓ表达的电泳检测、同位素标记、ＡＰ－Ｄ基因为探针的点印迹杂交、外源ＡＰ－Ｄ基因的ＰＣＲ扩增和Ｓｏｕｔｈｅｒｎ杂交。结果显示，报告基因ｎｏｓ、抗菌肽Ｄ基因已转入沙田柚植株细胞中。
Agrobacterium tumefaciens explants containing the nos gene and AP-D gene (including CaMV35s promoter) were synthesized and used to study the transgenic plants of Shatianyou grapefruit explants. The buds and seedlings produced by co-culture with Agrobacterium tumefaciens Km susceptibility screening. A number of transgenic plants were obtained, and the nos expression of the reporter gene was detected by electrophoresis, isotope labeling, dot-blot hybridization of AP-D gene probe and PCR amplification of exogenous AP-D gene And Southern hybridization. The results showed that the reporter gene nos, antimicrobial peptide D gene has been transferred to Shatin pomelo plant cells.