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目的:利用生物活体发光技术探讨寒凝血瘀证及热毒血瘀证对BALB/c小鼠4T1乳腺癌肺转移的影响。方法:21只雌性BALB/c小鼠分为3组:单纯荷瘤组、寒凝血瘀荷瘤组及热毒血瘀荷瘤组,在中医证候造模结束后分别原位接种4T1-luc乳腺癌细胞,测定各组小鼠原位肿瘤体积和体质量,并用活体生物发光成像仪动态观察各组小鼠乳腺癌肺转移情况。结果:在中医证候造模过程中,第7天时,热毒血瘀荷瘤组体质量较单纯荷瘤组及寒凝血瘀荷瘤组显著减轻(P<0.01,P<0.05),但随着时间推移,上述差异逐渐减小。荷瘤后,3组小鼠在原发肿瘤的生长体积方面无显著性差异。肺转移灶方面,3组在荷瘤的第21天无显著性差异;在荷瘤第28天,热毒血瘀荷瘤组的肺转移灶光子量数值最高,与另两组比较,差异显著(P<0.01)。结论:热毒血瘀证与4T1乳腺癌肺转移呈正相关,能促进4T1乳腺癌肺转移。
OBJECTIVE: To investigate the effect of cold-coagulation and blood-stasis syndrome and heat-toxic and blood-stasis syndrome on lung metastasis of 4T1 breast cancer in BALB / c mice by using bioluminescence technique. Methods: Twenty-one female BALB / c mice were divided into 3 groups: simple tumor-bearing group, cold coagulation and blood stasis tumor group and heat-toxic and blood stasis tumor group. After inoculation of TCM syndromes, 4T1-luc Breast cancer cells. The in situ tumor volume and body weight of mice in each group were measured. The lung metastasis of breast cancer in each group was observed dynamically by living bioluminescence imager. Results: During the process of TCM syndrome modeling, on the 7th day, the body mass of the heat toxin and blood stasis tumor group was significantly lower than that of the simple tumor and blood stasis group (P <0.01, P <0.05) Over time, the above differences gradually diminish. After tumor-bearing, there was no significant difference in the growth volume of the primary tumor in the three groups of mice. There was no significant difference in lung metastases between the three groups on the 21st day of tumor-bearing; in the 28th day of tumor-bearing, the photon quantity of lung metastases in the heat-toxic and stasis-bearing tumor group was the highest, which was significantly different from the other two groups (P <0.01). Conclusion: The heat-toxic and blood-stasis syndrome is positively correlated with lung metastasis of 4T1 breast cancer and can promote pulmonary metastasis of 4T1 breast cancer.