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弓形杆菌属(Arobacter sp.)和梭菌属(Clostridium sp.)广泛存在于水体和寄生在动物体内,极易造成水体污染和人畜共患病。采用高通量454焦磷酸测序方法对宁波沿海2个重点排污口、8个一般排污口的20个站位水样进行分析,得到1年中的3月、5月、8月和10月份各排污口弓形杆菌属和梭菌属生物的分布情况。研究结果表明:弓形杆菌属检测出两个种,分别是嗜低温弓形杆菌(Arcobacter cryaerophilus)、布氏弓形杆菌(Arcobacter butzleri)。梭菌属共检测出12个种,其中产气荚膜梭菌(Clostridium perfringens)检出次数最高,丁酸梭菌(Clostridium butyricum)次之;排污口的类型是影响弓形杆菌属检出量的重要因素。弓形杆菌属主要分布在氮,磷含量较高的工业排污口和市政排污口,而梭菌属只在3月份的S7宁海西店崔家综合排污口呈现出相对较高的比例(7.5%),其它排污口检出量均在2%以下;弓形杆菌属和梭菌属在10个排污口20个站位4个月份的检出次数呈现正相关性;由季节性的变化引起的气温差异也是影响弓形杆菌属生长的重要因素,弓形杆菌属在5月份的时候检出次数最大,梭菌属适宜生长温度范围较广10—65°C,受温度影响较小。
Arobacter sp. And Clostridium sp. Exist widely in water and parasitically in animals and can easily cause water pollution and zoonosis. The high-throughput 454 pyrosequencing method was used to analyze the water samples of 20 stations along the two major sewage outfalls and 8 general outfalls along the coast of Ningbo. The water samples of March, May, August and October in each year The distribution of Arcobacter spp. And Clostridium spp. The results showed that Arcobacter genus and Arcobacter butzleri were detected in Arcobacter genus. Clostridium detected a total of 12 species, which Clostridium perfringens detected the highest number of Clostridium butyricum (Clostridium butyricum) followed; the type of sewage outfall is the impact of detection of Arcobacterium Key factor. Arcobacter genus is mainly distributed in industrial sewage outfalls and municipal sewage outfalls with high content of nitrogen and phosphorus, while Clostridium only shows a relatively high proportion (7.5%) in Cui comprehensive outfall at S7 Ninghaixi store in March, , The other outfall detection volume are below 2%; Arcobacter and Clostridium in 10 outfall 20 stations in 4 months the number of positive correlation was detected; seasonal changes caused by temperature differences Arcobacter also affect the growth of important factors, Arcobacterium detected in May the largest number of Clostridium suitable growth temperature range of 10-65 ° C, less affected by temperature.