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通过向大鼠外侧缰核微电泳乙酰胆碱（Ａｃｈ）０．４ｍｏｌ观察到，Ａｃｈ对大多数缰核神经元的自发放电表现为兴奋作用。通过实验还观察到，电针足三里穴使痛阈增高，缰核内微量注射Ａｃｈ可显著地拮抗电针镇痛。
Ach stimulation of spontaneous discharge in most of the habenular nucleus neurons was observed by observing 0.4 mol of acetylcholine (Ach) to the rat lateral habenular nucleus. The experiment also observed that electroacupuncture at Zusanli acupoint increased the threshold of pain. Microinjection of Ach into the habenula markedly antagonized the electroacupuncture analgesia.