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目的：了解丹参对体外培养的成骨细胞生长、分化与代谢的影响．方法：选择克隆化成骨细胞株ＭＣ３Ｔ３－Ｅ１细胞，采用细胞培养方法，观察细胞内ＤＮＡ合成与碱性磷酸酶（ＡＬＰａｓｅ）活性．结果：丹参明显增强ＭＣ３Ｔ３－Ｅ１细胞内ＡＬＰａｓｅ活性，丹参浓度为５．０ｇ／Ｌ时，对ＭＣ３Ｔ３－Ｅｌ细胞内ＡＬＰａｓｅ活性影响最大，比对照组强约１３５％。丹参这种促进作用只限于分化晚期的ＭＣ３Ｔ３－Ｅ１细胞，而对分化早期细胞内的ＡＬＰａｓｅ活性起抑制作用，其次，这种作用还与药物作用时间有关．但是，丹参对各生长分化期的ＭＣ３Ｔ３－Ｅ１细胞内ＤＮＡ合成均无影响。结论：丹参可明显增强体外培养分化晚期ＭＣ３Ｔ３－Ｅ１细胞内的ＡＬＰａｓｅ活性，该作用不是通过促进细胞增殖，而是改善细胞功能而实现的，并且受丹参药物浓度与作用时间的影响，说明丹参可能在正畸牙齿移动中促进新生牙槽骨的形成方面发挥重要作用。
Objective: To understand the effects of salvia miltiorrhiza on the growth, differentiation and metabolism of osteoblasts cultured in vitro. METHODS: The cloned osteoblast MC3T3-E1 cells were selected and cell culture was used to observe the intracellular DNA synthesis and alkaline phosphatase (ALPase) activity. Results: Salvia miltiorrhiza significantly enhanced the ALPase activity in MC3T3-E1 cells. When the concentration of Salvia miltiorrhiza was 5.0 g/L, the ALPase activity in MC3T3-El cells had the greatest effect, which was about 135% stronger than that of the control group. The promotion effect of Salvia miltiorrhiza is limited to MC3T3-E1 cells in the late stage of differentiation, but it inhibits the ALPase activity in cells during the early stage of differentiation. Second, this effect is also related to the time of drug action. However, Salvia miltiorrhiza has no effect on DNA synthesis in MC3T3-E1 cells at different growth stages. Conclusion: Salvia miltiorrhiza can obviously enhance the ALPase activity in MC3T3-E1 cells differentiated in vitro. This effect is not achieved by promoting cell proliferation, but by improving cell function. It is also affected by the drug concentration and action time of Salvia miltiorrhiza. It plays an important role in promoting the formation of new alveolar bone during orthodontic tooth movement.